Details

Name
Vessel Proteoglycan Analysis
IQbot ID
IQBOT-0003
Version
1.0.0
Research Area(s)
Cardiovascular, Drug Toxicity & Metabolism
Processing Time Expectation
Less than 4 Minutes per Image
Acquisition Hardware
Bright-Field Microscopy
Techniques, Dyes, Stains, & Proteins
Movat Pentachrome
Sample Context
In Tissue
Name
Vessel Proteoglycan Analysis
IQbot ID
IQBOT-0003
Version
1.0.0
Research Area(s)
Cardiovascular, Drug Toxicity & Metabolism
Processing Time Expectation
Less than 4 Minutes per Image
Acquisition Hardware
Bright-Field Microscopy
Techniques, Dyes, Stains, & Proteins
Movat Pentachrome
Sample Context
In Tissue

Output Parameters

Vessel Area (mm²) 
The total area, measured in square millimeters, of the vessels sectioned in each image.
Vessel Mean Diameter (mm) 
The average diameter, measured in millimeters, of the vessels sectioned in each image.
Vessel Maximum Diameter (mm) 
The largest measured diameter of the vessels sectioned in each image, reported in millimeters.
Vessel Minimum Diameter (mm) 
The smallest measured diameter of the vessels sectioned in each image, reported in millimeters.
Vessel Perimeter (mm) 
The path length of the perimeter of the vessels sectioned in each image, reported in millimeters.
Lumen Area (mm²) 
The total area, measured in square millimeters, of the vessel lumen in each image.
Lumen Mean Diameter (mm) 
The average diameter, measured in millimeters, of the vessel lumen in each image.
Lumen Maximum Diameter (mm) 
The largest measured diameter of the vessel lumen in each image, reported in millimeters.
Lumen Minimum Diameter (mm) 
The smallest measured diameter of the vessel lumen in each image, reported in millimeters.
Lumen Perimeter (mm) 
The path length of the perimeter of the vessel lumen in each image, reported in millimeters.
Media Area (mm²) 
The total area, measured in square millimeters, of the vessel media in each image.
Media Mean Thickness (mm) 
The average thickness, measured in millimeters, of the vessel media in each image.
Media Maximum Thickness (mm) 
The largest measured thickness of the vessel media in each image, reported in millimeters.
Media Minimum Thickness (mm) 
The smallest measured thickness of the vessel media in each image, reported in millimeters.
Media Standard Deviation of Thickness (mm) 
The standard deviation of thickness measurements made along the entire vessel media in each image, reported in millimeters.
Media Proteoglycan Area (mm²) 
The area within the media, in square millimeters, occupied by proteoglycans.
Neointima Area (mm²) 
The total area, measured in square millimeters, of the vessel neointima in each image.
Neointima Mean Thickness (mm) 
The average thickness, measured in millimeters, of the vessel neointima in each image.
Neointima Maximum Thickness (mm) 
The largest measured thickness of the vessel neointima in each image, reported in millimeters.
Neointima Minimum Thickness (mm) 
The smallest measured thickness of the vessel neointima in each image, reported in millimeters.
Neointima Standard Deviation of Thickness (mm) 
The standard deviation of thickness measurements made along the entire vessel neointima in each image, reported in millimeters.
Neointima Proteoglycan Area (mm²) 
The area within the neointima, in square millimeters, occupied by proteoglycans.

 

This section will identify any output parameters that are only output by the IQbot in its advanced configuration.

 

Vessel Area (mm²) 
The total area, measured in square millimeters, of the vessels sectioned in each image.
Vessel Mean Diameter (mm) 
The average diameter, measured in millimeters, of the vessels sectioned in each image.
Vessel Maximum Diameter (mm) 
The largest measured diameter of the vessels sectioned in each image, reported in millimeters.
Vessel Minimum Diameter (mm) 
The smallest measured diameter of the vessels sectioned in each image, reported in millimeters.
Vessel Perimeter (mm) 
The path length of the perimeter of the vessels sectioned in each image, reported in millimeters.
Lumen Area (mm²) 
The total area, measured in square millimeters, of the vessel lumen in each image.
Lumen Mean Diameter (mm) 
The average diameter, measured in millimeters, of the vessel lumen in each image.
Lumen Maximum Diameter (mm) 
The largest measured diameter of the vessel lumen in each image, reported in millimeters.
Lumen Minimum Diameter (mm) 
The smallest measured diameter of the vessel lumen in each image, reported in millimeters.
Lumen Perimeter (mm) 
The path length of the perimeter of the vessel lumen in each image, reported in millimeters.
Media Area (mm²) 
The total area, measured in square millimeters, of the vessel media in each image.
Media Mean Thickness (mm) 
The average thickness, measured in millimeters, of the vessel media in each image.
Media Maximum Thickness (mm) 
The largest measured thickness of the vessel media in each image, reported in millimeters.
Media Minimum Thickness (mm) 
The smallest measured thickness of the vessel media in each image, reported in millimeters.
Media Standard Deviation of Thickness (mm) 
The standard deviation of thickness measurements made along the entire vessel media in each image, reported in millimeters.
Media Proteoglycan Area (mm²) 
The area within the media, in square millimeters, occupied by proteoglycans.
Neointima Area (mm²) 
The total area, measured in square millimeters, of the vessel neointima in each image.
Neointima Mean Thickness (mm) 
The average thickness, measured in millimeters, of the vessel neointima in each image.
Neointima Maximum Thickness (mm) 
The largest measured thickness of the vessel neointima in each image, reported in millimeters.
Neointima Minimum Thickness (mm) 
The smallest measured thickness of the vessel neointima in each image, reported in millimeters.
Neointima Standard Deviation of Thickness (mm) 
The standard deviation of thickness measurements made along the entire vessel neointima in each image, reported in millimeters.
Neointima Proteoglycan Area (mm²) 
The area within the neointima, in square millimeters, occupied by proteoglycans.

Image Acquisition


This IQbot was developed to analyze images captured with a bright-field microscope in vessel cross-sections that have been stained with Movat Pentachrome. Sections imaged and subsequently submitted for analysis should depict the entire vessel lumen, neointima, and media.

 
 

The Vessel Proteoglycan Analysis IQbot has been built to accommodate the following imaging types only.

   .tif 
  .tiff
  .bmp
 .jpg
.jpeg
 
 

As we reference for IQbots in general here, the Vessel Proteoglycan Analysis is only ultimately effective if you upload high quality images for processing. Please ensure that any images you upload follow the below guidelines specific to this IQbot.

High-Resolution:  The quality of the output data you receive is largely dependent on the input image resolution. Please provide high-resolution images to ensure that the IQbot can most effectively differentiate between features.
In-Focus:  Please produce the most in-focus images possible for analysis. Images with high background contrast will result in more accurate and useful data and overlays.
Properly Saturated:  If your submitted images are significantly undersaturated or oversaturated, it is possible that the IQbot will be unable to detect structures, or may group structures together. Please ensure that the images you upload are saturated appropriately.
Clear of Artifacts:  Please take care to avoid creating bubbles, debris, or otherwise inducing artifacts into your image. Such additional features may corrupt the resulting data.
Clear of Accessary Information:  Please do not submit images retaining any annotations, legends, timestamps, or other markings. Every part of the images that you upload will be analyzed and included in the output data.
 
 
 

If you are uncertain whether or not your images meet the quality standards for which this IQbot was validated, or have additional questions relating to image quality, please view our FAQs or contact the ImageQuantify.com support team.

 

     (216) 678-9258           help@imagequantify.com

 

Validation Information


This IQbot has been successfully validated for use, and a copy of its validation certificate accompanies the results of each order it analyzes. In accordance with general ImageQuantify.com validation procedures, this IQbot has been found to provide quantitative information equivalent to that of a manual observer, but with no variability and increased breadth of information.

 
 

The first step of the validation process was to establish the ground truth against which the IQbot’s efficacy would be compared. For the purpose of the Vessel Proteoglycan Analysis IQbot, ground truth was defined on a manual observer basis. Trained observers were guided through a standardized, semi-automated process to produce output data from a specially selected validation image set. This image set represented the entire range of expected output values (e.g., diverse placement and density of proteoglycan presence). Images were presented for analysis in a randomized order and deidentified to protect against observer biases. The observer mean for each parameter measured was accepted as ground truth.

By analyzing the inter- and intraobserver variability present in the manual observation process, acceptable criteria were established so as to compare the IQbot’s output to that of a trained replacement observer. Ultimately, the IQbot’s performance proved to offer significant upgrades over manual or semi-automated analysis techniques, and validation was considered successful. Further considerations ensured that the IQbot rejected and flagged concerning image-specific outputs and performed within operation time constraints.

 

The Vessel Proteoglycan Analysis IQbot evaluates proteoglycan content in images of Movat Pentachrome stained vessel cross-sections acquired using brightfield microscopy. The IQbot provides the area, perimeter, and diameter (min, max, and mean) of the vessel and lumen, the thickness (min, max, mean, and standard deviation) and area of the neointima and media, and the area and percent coverage of proteoglycan in both the media and neointima. Finally the IQbot, pseudo-colors and superimposes the contours of the vessel, media inner wall, and lumen as well as the segmented proteoglycan rich regions upon the original image.

DEMO IMAGE
IQBOT APPLIED IMAGE

 

SAMPLE DATA
Image Vessel Area
(mm²)
Vessel Mean Diameter
(mm)
Vessel Maximum Diameter
(mm)
Vessel Minimum Diameter
(mm)
Vessel Perimeter
(mm)
Lumen Area
(mm²)
Lumen Mean Diameter
(mm)
Lumen Maximum Diameter
(mm)
Lumen Minimum Diameter
(mm)
Lumen Perimeter
(mm)
Media Area
(mm²)
Media Mean Thickness
(mm)
Media Maximum Thickness
(mm)
Media Minimum Thickness
(mm)
Media Standard Deviation of Thickness
(mm)
Media Proteoglycan Area
(mm²)
Neointima Area
(mm²)
Neointima Mean Thickness
(mm)
Neointima Maximum Thickness
(mm)
Neointima Minimum Thickness
(mm)
Neointima Standard Deviation of Thickness
(mm)
Neointima Proteoglycan Area
(mm²)
Sample 3.779089 2.190659 2.340791 2.011527 7.084886 1.717498 1.467470 1.705971 1.250355 4.983283 1.954824 0.320638 0.436736 0.252488 0.040905 0.250874 0.106767 0.018601 0.078476 0.005118 0.010621 0.006516